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【技术文章】miRNA功能研究整体解决方案

发布时间:2020-06-02 阅读次数:46

MicroRNA (miRNA)是从真核基因组转录形成的非编码单链小分子RNA。它们的序列高度保守,长度通常是21到23个核苷酸。近来研究发现miRNA几乎与所有细胞的功能和生物体生命过程有关。它们通过靶定到mRNA3’非翻译区(3'-UTR)来调节基因的表达。MicroRNA所调节基因的表达失调与癌症、心血管疾病等各种疾病有关。

 

GeneCopoeia提供microRNA (miRNA)完整研究方案,帮助客户检测、验证或敲低miRNA的表达,覆盖miRBase中人、小鼠、大鼠 miRNA。


一、相关研究的miRNA的筛选———筛选感兴趣的miRNA 


 miRNA qPCR arrays


Cat. No. Array type Number of
miRNAs
Number of plates
PAM-HG96 Human miRNome 1,565 19 x 96-well plates
PAM-HC96 Human cancer 520 5 x 96-well plates
PAM-HCN96 Human brain cancer 84 1 x 96-well plate
PAM-HCB96 Human breast cancer 168 2 x 96-well plates
PAM-HCX96 Human leukemia 168 2 x 96-well plates
PAM-HCL96 Human lung cancer 168 2 x 96-well plates
PAM-HCO96 Human ovarian cancer 168 2 x 96-well plates
PAM-HCY96 Human bladder cancer 79 1 x 96-well plate
PAM-HCC96 Human colorectal cancer 84 1 x 96-well plate
PAM-HCE96 Human endometrial cancer 84 1 x 96-well plate
PAM-HCG96 Human gastric cancer 80 1 x 96-well plate
PAM-HCH96 Human hepatocellular carcinoma 168 2 x 96-well plates
PAM-HCZ96 Human lymphoma 84 1 x 96-well plate
PAM-HCM96 Human melanoma 84 1 x 96-well plate
PAM-HCT96 Human head and neck cancer 84 1 x 96-well plate
PAM-HCP96 Human pancreatic cancer 84 1 x 96-well plate
PAM-HCQ96 Human prostate cancer 84 1 x 96-well plate
PAM-HI96 Human immunopathology 84 1 x 96-well plate
PAM-HX96 Human serum and Plasma 168 2 x 96-well plates
PAM-HF96 Human inflammatory 84 1 x 96-well plate
PAM-HH96 Human heart disease 84 1 x 96-well plate
PAM-HM96 Human muscle disease 84 1 x 96-well plate
PAM-HT96 Human toxicology related 84 1 x 96-well plate
PAM-HK96 Human IPS (stem cell) 168 2 x 96-well plates


二、通过克隆或者miRNA模拟物(mimics),实现调控miRNA表达量的高低


miRNA相关克隆


三、准确定量相应miRNA表达量以及目的基因表达量

miRNA primers and kits


 

Catalog# Product Description
QP013 All-in-One™ miRNA First-Strand cDNA
Synthesis Kit (20 RT reactions)
Poly A Polymerase, RTase Mix, PAP/
RT buffer, spike-in control (for use with
miRNA qPCR arrays)
QP014 All-in-One™ miRNA First-Strand cDNA
Synthesis Kit (60 RT reactions)
Poly A Polymerase, RTase Mix, PAP/
RT buffer, spike-in control (for use with
miRNA qPCR arrays)
QP015 All-in-OnemiRNA qRT-PCR Detection Kit
(20 RT and 200 qPCR reactions)
Poly A polymerase, RTase mix, qPCR
mix, ROX reference dye, universal
adaptor PCR primer and other buffers
QP016 All-in-OnemiRNA qRT-PCR Detection Kit
(60 RT and 600 qPCR reactions)
Poly A polymerase, RTase mix, qPCR
mix, ROX reference dye, universal
adaptor PCR primer and other buffers


四、双荧光素酶报告验证miRNA分子作用机制

miRNA Assays






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